Feeds:
Posts
Comments

Posts Tagged ‘cucumber mosaic virus’

Science for Environment Policy

European Commission

http://ec.europa.eu/environment/integration/research/newsalert/pdf/controlling_resilient_plant_viruses_with_steam_404na3_en.pdf

Combining steam with heat-producing chemicals could control pathogenic
viruses in soil, finds new research. The study examined how effective different
forms of heat sterilisation of soil were at inactivating three plant viruses. While
steam alone was enough to eradicate two of the viruses, the highly resilient
tobacco mosaic virus required the addition of exothermic chemicals to reduce
it by 97%.

——————————————————————————————————-
Injecting the chemical methyl bromide into soils, which can be carried out on large soilsurfaces, was a highly effective method of sterilising soils, and killing off pests. However,while methyl bromide has been widely used — and still is in countries outside Europe — for this purpose, as well as to fumigate wood products and packaging, it is also an ozone depleting and poisonous chemical now controlled under the Montreal Protocol. As such, its use is being phased out, although there have still been some successful instances of lobbying for critical-use exemptions after the Protocol’s 2010 deadline.

Among the possible alternatives is heat sterilisation, using steam or ‘solarisation’. Steam sterilisation involves the active injection of steam into soils over less than an hour, while solarisation uses the sun to passively heat soil underneath a transparent covering. Both methods heat the soil sufficiently to kill a range of pathogens. However, these approaches have not been evaluated for their ability to control resilient viruses, such as the tobacco mosaic virus.

his research examined how effective combining soil steaming with exothermic chemicals was at controlling three different plant viruses of commercial importance. The effectiveness of solarisation, with coverings with different thermal properties, was also tested. Mesh bags containing soil mixed with three plant viruses — tobacco mosaic virus, potato virus Y and cucumber mosaic virus — were placed in soil at depths of 20 cm and 40 cm. Soil solarisation was tested using three different types of plastic films: transparent polyethylene, ethylene-vinyl acetate and an ‘infrared film’. The films were used to cover the soil for 20 days during July. The researchers also set up a control plot, without any covering.

Solarisation did not have any effect on any of the viruses at 40 cm depth, compared with the uncovered control plot, and the tobacco mosaic virus was unaffected even at 20 cm. The cucumber mosaic virus was eliminated at 20 cm depth in all solarisation plots, but this was also true for the control plot. The potato virus Y was completely eradicated at 20 cm by ethylene-vinyl acetate and infrared film coverings.

The researchers also carried out soil steaming either with just steam or using potassium hydroxide or calcium oxide, which both release heat when exposed to water. Following steam treatments, neither potato virus Y nor cucumber mosaic virus were capable of causing infections, regardless of depth or chemical used. Adding the chemicals caused a significant decrease in the ability of the tobacco mosaic virus to cause infections,  from around 50–60% to below 3%.

The researchers also tested solarised and steamed soil to see how either treatment affected the microbial communities (e.g. bacteria and fungi) in soil, some of which can be beneficial for growing plants. They found that while both treatments reduced the amount of microbes (microbial biomass); the diversity of microbes appeared to remain the same as in untreated soils.

Tobacco mosaic virus is an extensively studied ‘model’ virus known to be an extremely hardy and difficult to control. As such, the finding that adding exothermic chemicals to steam treatments may offer a new way to control it, and could indicate a potentially less poisonous method in the control of similarly hardy agricultural pests.

Source: Luvisi, A.,
Panattoni, A., & Materazzi,
A. (2014). Heat treatments
for sustainable control of
soil viruses. Agronomy for
Sustainable Development.
DOI:10.1007/s13593-014-
0258-x
Contact:
aluvisi@agr.unipi.it

Read Full Post »

Phys.Org

Aug 22,2014

http://phys.org/news/2014-08-virus-dna.html

dnaplantscanswi

A team of virologists and plant geneticists at Wageningen UR has demonstrated that when tomato plants contain Ty-1 resistance to the important Tomato yellow leaf curl virus (TYLCV), parts of the virus DNA (the genome) become hyper-methylated, the result being that virus replication and transcription is inhibited. The team has also shown that this resistance has its Achilles heel: if a plant is simultaneously infected with another important (RNA) virus, the Cucumber mosaic virus (CMV), the resistance mechanism is compromised.

Antiviral defence via RNAi
Plant defence to viruses usually depends on RNA interference (RNAi). The genetic material of many viruses consists of RNA. A complex process in the plant causes the virus RNA to be chopped up into pieces, which means the virus can no longer multiply. In contrast to most other disease-causing plant viruses, the genetic material in TYLCV is DNA, not RNA. Therefore antiviral RNAi defence to these viruses has to happen somewhat different.

TYLCV is one of the most economically important plant viruses in the world; for this virus a number of resistance genes (Ty-1 to Ty-6) are available to commercial plant breeders. In 2013 the researchers in Wageningen succeeded in identifying and cloning the Ty-1 gene, which happened to present a member from an important class of RNAi-pathway genes. This led to a publication in PLoS Genetics. Their recent publication in the journal PNAS shows that although Ty-1 resistance depends on RNAi, instead of the genetic material being chopped up, it is being ‘blocked’ by methylation of the virus DNA.

No cross protection
A well-known phenomenon in the plant world is the ‘immunisation’ of plants by infecting them with relatively harmless viruses. The latter ensures that the defence mechanisms in plants are activated and provide ‘cross protection’ against more harmful, related viruses.

To their great surprise, the Wageningen researchers discovered that infection with CMV, a virus that contains RNA as genetic material and that, as a result, is not affected by the Ty-1 resistance mechanism, actually compromised resistance to the TYLCV virus. According to the researchers, this is a warning to plant breeders. The use of the Ty-1 gene does provide resistance, but the mechanism will be at risk in plants grown in greenhouses and fields if the plants are attacked by various other types of viruses.

Explore further: Virus rounds up enzymes, disarms plant
More information: Patrick Butterbach, Maarten G. Verlaan, Annette Dullemans, Dick Lohuis, Richard G. F. Visser, Yuling Bai, and Richard Kormelink. “Tomato yellow leaf curl virus resistance by Ty-1 involves increased cytosine methylation of viral genomes and is compromised by cucumber mosaic virus infection.” PNAS 2014 ; published ahead of print August 18, 2014, DOI: 10.1073/pnas.1400894111
Journal reference: PLoS Genetics Proceedings of the National Academy of Sciences
Provided by Wageningen University

Read more at: http://phys.org/news/2014-08-virus-dna.html#jCp

Read Full Post »