Development of Powdery Mildew Resistant Tomato via CRISPR-Cas9
The team targeted the SlMlo1 locus using the double sgRNA strategy. Transformants were analyzed and eight out of ten tested T0 transformants indicated the presence of mutations. Assays using the powdery mildew fungus revealed that all the generated T0 slmlo1 mutant plants were resistant to the pathogen, while wild-type plants were susceptible. Furthermore, the slmlo1 mutant plants were morphologically similar to the wild type and also produced harvested fruit weight similar to the wild types. The team named the generated variety Tomelo. This study presents evidence for CRISPR-Cas9 being a highly precise tool for genome editing in tomato. For more on this study, read the article in Nature. |
Powdery Mildew Resistant Tomato via CRISPR-Cas9
July 13, 2017 by IAPPS
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